Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10847847 | Steroids | 2005 | 8 Pages |
Abstract
We report a novel conjugate, bile acid acyl galactosides, which exist in the urine of healthy volunteers. To identify the two unknown peaks obtained in urine specimens from healthy subjects, the specimens were subjected to solid phase extraction and then to liquid chromatographic separation. The eluate corresponding to the unknown peaks on the chromatogram was collected. Following alkaline hydrolysis and liquid chromatography (LC)/electrospray ionization (ESI)-mass spectrometric (MS) analysis, cholic acid (CA) and deoxycholic acid (DCA) were identified as liberated bile acids. When a portion of the alkaline hydrolyzate was subjected to a derivatization reaction with 1-phenyl-3-methyl-5-pyrazolone, a derivative of galactose was detected by LC/ESI-MS. Finally, the liquid chromatographic and mass spectrometric properties of these unknown compounds in urine specimens were compared to those of authentic specimens and the structures were confirmed as CA 24-galactoside and DCA 24-galactoside. These results strongly imply that bile acid 24-galactosides, a novel conjugate, were synthesized in the human body.
Keywords
DCAHDCACDCAUGTUDCAAPCIPMP3-Methyl-1-phenyl-5-pyrazoloneESIMS/MSLCAChenodeoxycholic acidDeoxycholic acidBile acidLithocholic acidHyodeoxycholic acidCholic acidUrsodeoxycholic acidMass spectrometryTandem mass spectrometryliquid chromatographyLiquid chromatography/mass spectrometryGalactoseGlycosideelectrospray ionisationelectrospray ionizationatmospheric pressure chemical ionizationAtmospheric pressure chemical ionisation
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Authors
Takaaki Goto, Akihiro Shibata, Daisuke Sasaki, Naoto Suzuki, Takanori Hishinuma, Genta Kakiyama, Takashi Iida, Nariyasu Mano, Junichi Goto,