| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889526 | Journal of Microbiological Methods | 2013 | 4 Pages |
Abstract
A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.
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Authors
Lei Tan, Hongjun Chen, Shengqing Yu, Xusheng Qiu, Cuiping Song, Danqing Chen, Shilei Zhang, Fanqing Zhang, Suibin He, Xinyue Shen, Meirong Hu, Chan Ding,