| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889544 | Journal of Microbiological Methods | 2013 | 5 Pages |
Abstract
⺠A new and efficient protocol for gene replacement in intact B. subtilis was developed. ⺠It is based on endogenous HR machinery and full ssDNA constructs as direct substrate. ⺠We showed that ssDNA is advantageous over dsDNA to stimulate HR in intact B. subtilis. ⺠Increased HR frequency correlates with lagging-strand bias and 5Ⲡend protection. ⺠A lipase gene was successfully integrated and expressed by applying this strategy.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Sai Wen, Jianguo Yang, Tianwei Tan,