| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889548 | Journal of Microbiological Methods | 2013 | 8 Pages |
Abstract
⺠We designed four Sclerotinia species specific end-point PCR primer sets. ⺠Our primers are compatible with multiplex-PCR. ⺠Primer specificity and sensitivity were tested individually and in multiplex. ⺠Diagnostic assays were validated using genomic DNA from fungi and infected plants. ⺠Our multiplex-PCR assay accurately discriminates four Sclerotinia species.
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Authors
Ahmed Abd-Elmagid, Patricia A. Garrido, Robert Hunger, Justin L. Lyles, Michele A. Mansfield, Beth K. Gugino, Damon L. Smith, Hassan A. Melouk, Carla D. Garzon,