Simple and rapid discrimination of embB codon 306 mutations in Mycobacterium tuberculosis clinical isolates by a real-time PCR assay using an LNA-TaqMan probe

► We develop a rapid and simple real-time PCR assay for discriminating embB306 SNPs. ► This assay uses a LNA-TaqMan probe instead of a normal DNA-TaqMan probe. ► This system requires only less than 0.1 pg of genomic DNA per reaction. ► Mutant is easily distinguishable by its amplification curve without melting analysis.