| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889552 | Journal of Microbiological Methods | 2013 | 7 Pages |
Abstract
⺠A one-step multiplex PCR method employing 12 SSR markers was developed for fast genotyping of Phytophthora infestans. ⺠This one-step multiplex PCR method resolved distinct multilocus genotypes (MLGs) of isolates around the globe. ⺠Standardization with guidelines and reference material allowed unambiguous scoring of more than 5000 isolates. ⺠The methods presented allow the analysis of datasets comprising isolates of mixed ploidy levels in P. infestans. ⺠Two methods were developed for direct P. infestans genotyping from infected leaves and stored infected leaf extracts.
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Authors
Ying Li, David E.L. Cooke, Evert Jacobsen, Theo van der Lee,