| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889569 | Journal of Microbiological Methods | 2013 | 7 Pages |
Abstract
A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.
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Authors
Ewa Pacholewicz, Arno Swart, Len J.A. Lipman, Jaap A. Wagenaar, Arie H. Havelaar, Birgitta Duim,