| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889581 | Journal of Microbiological Methods | 2013 | 5 Pages |
Abstract
⺠Propidium monoazide (PMA) was applied in combination with quantitative PCR (qPCR) for selective detection of intact cells. ⺠Co-incubation of PMA with deoxycholate can only be applied to gram-negative species. ⺠PMA incubation temperature has a strong effect on dye uptake by damaged cells. ⺠When working with low dye concentrations, PMA-qPCR efficiency can easily be improved by longer incubation times. ⺠Elevated dye incubation temperature and extended incubation time greatly improve exclusion of dead cell signals.
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Authors
Esther Nkuipou-Kenfack, Holger Engel, Sarah Fakih, Andreas Nocker,