Article ID Journal Published Year Pages File Type
10889614 Journal of Microbiological Methods 2012 4 Pages PDF
Abstract
► Effects of LNA substitution on topoisomerase cloning of amplicons are described. ► 3′ overhangs were missing in Taq-amplified PCR products. ► Topoisomerase-based cloning efficiency was affected in a sequence dependent manner. ► Topoisomerase cloning of LNA-substituted amplicons is prone to cloning bias.
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Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Preview
Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers
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