| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889631 | Journal of Microbiological Methods | 2012 | 9 Pages |
Abstract
⺠Provide guidelines for standardized microbiome-based studies of house dust. ⺠DNA yield, 16S rRNA dose response and assay reproducibility were tested. ⺠Pilot study revealed hierarchy in community composition concordance. ⺠Least community variability between technical replicates, greatest between rooms from different houses. ⺠454-Pyrosequencing verified bacteria detected by phylogenetic microarray.
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Authors
Kei E. Fujimura, Marcus Rauch, Elizabeth Matsui, Shoko Iwai, Agustin Calatroni, Henry Lynn, Herman Mitchell, Christine C. Johnson, James E. Gern, Alkis Togias, Homer A. Boushey, Suzanne Kennedy, Susan V. Lynch,