Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes

Article ID	Journal	Published Year	Pages	File Type
10889637	Journal of Microbiological Methods	2012	7 Pages	PDF

Abstract

âº Multiplex PCR was used to detect C. difficile virulence genes. âº Detection over a wide temperature range enabled sequence analysis. âº Mismatch-tolerant probes were used to distinguish strains. âº Three probes with the same fluorophore identified tcdC sequence variations. âº Signal ratios distinguished variations independent of PCR product concentration.

Keywords

Multiplex PCR Hybridization probes Clostridium difficile

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology

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Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes

Authors

Kenneth E. Pierce, Huma Khan, Rohit Mistry, Simon D. Goldenberg, Gary L. French, Lawrence J. Wangh,