| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889642 | Journal of Microbiological Methods | 2012 | 6 Pages |
Abstract
⺠A novel vector family for chromosomal integration in B. subtilis was constructed. ⺠Unique restriction sites allow simple exchange of all modules. ⺠Untagged or strep-tagged proteins can be expressed under single-copy conditions. ⺠Constitutive or inducible promoters and a promoterless lacZ gene are included. ⺠pMG plasmids show in contrast to pBR322-based vectors no structural instability.
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Authors
Matthias Gimpel, Sabine Brantl,