Article ID Journal Published Year Pages File Type
10889644 Journal of Microbiological Methods 2012 8 Pages PDF
Abstract
► An improved Nile red assay for estimation of intracellular yeast lipid is developed. ► The use of 96-well microplate is high-throughput for screening large sample number. ► No prior cell washing with buffer is needed. ► Dimethyl sulfoxide improves Nile red staining for certain species. ► Kinetic reading allows different yeast strains to reach maximum emission.
Keywords
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Preview
An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species
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