| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889649 | Journal of Microbiological Methods | 2012 | 8 Pages |
Abstract
⺠We describe the creation of a novel yeast two-hybrid strain, BAPJ69-4A. ⺠BAPJ69-4A contains four markers: ADE2, HIS3, URA3, and lacZ. ⺠The addition of the URA3 marker allows for both positive and negative selection. ⺠The differences in marker stringency allow for highly tunable assay sensitivity. ⺠BAPJ69-4A was characterized using the retinoid X receptor and variants.
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Authors
Hally Anne Shaffer, Michael Kenneth Rood, Badar Kashlan, Eileen I-ling Chang, Donald Francis Doyle, Bahareh Azizi,