| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889699 | Journal of Microbiological Methods | 2011 | 5 Pages |
Abstract
⺠Biphasic blood-based SNB-9 medium has a large volume of the liquid phase. ⺠Large volume of the liquid phase facilitates the manipulation. ⺠SNB-9 provides high Leishmania yields sufficient for large-scale experiments. ⺠Parasites cultured in SNB-9 have a high in vitro infectivity.
Keywords
PBSHBSSDMEMFBSRT PCRSSCNNNFSCGFPDNADulbecco's modified Eagle's mediumdeoxyribonucleic acidstandard deviationELISAEnzyme-linked immunosorbent assayfetal bovine serumPhosphate buffered salineLeishmaniaHanks' balanced salt solutionpolymerase chain reactionReal time polymerase chain reactionPCRforward scatterside scattergreen fluorescent proteinPropidium iodide
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Authors
Igor Grekov, Milena Svobodová, Eva Nohýnková, Marie Lipoldová,