| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889755 | Journal of Microbiological Methods | 2011 | 5 Pages |
Abstract
⺠Sequencing of DNA submitted to Multiple Displacement Amplification (MDA) may preclude genome assembly. ⺠We extracted genomic V. cholerae DNA in agarose block to ensure circularity. ⺠We MDA amplified 10 ng and assembled genome sequence de novo. ⺠Chimeras and spurious sequences were minimal.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Paula Juliana Pérez Chaparro, John Anthony McCulloch, Louise Teixeira Cerdeira, Arwa Al-Dilaimi, Lena Lillian Canto de Sá, Rodrigo de Oliveira, Andreas Tauch, Vasco Ariston de Carvalho Azevedo, Maria Paula Cruz Schneider, Artur Luiz da Costa da Silva,