Differentiation among the Vibrio cholerae serotypes O1, O139, O141 and non-O1, non-O139, non-O141 using specific monoclonal antibodies with dot blotting

► MAbs specific to each pathogenic serogroup of V. cholerae O1, O139, O141or all isolates were produced. ► These MAbs can be used to detect V. cholerae with a sensitivity range from 105 to 107 CFU ml-1 using dot blotting. ► In complex samples, V. cholerae can be detected and differentiated without the requirement for bacterial isolation. ► These MAbs are potentially used for the development of a strip test for detection of various serogroups and all V. cholerae.