| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889775 | Journal of Microbiological Methods | 2011 | 7 Pages |
Abstract
⺠A quantitative and rapid detection method for rotavirus in water samples was developed using immunomagnetic separation combined with quantitative RT-PCR (IMS-RT-qPCR). ⺠Compared to extracting RNA using commercial kits, the IMS increased the detection sensitivity by about one order of magnitude from clean water. ⺠The IMS-RT-qPCR was also successfully applied to purify and detect rotavirus seeded in 103-fold concentrated wastewater influents. ⺠The results of present study indicated that IMS-RT-qPCR is a rapid, sensitive and reliable tool for detecting rotaviruses in complex water environments.
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Authors
Wan Yang, April Z. Gu, Si-yu Zeng, Dan Li, Miao He, Han-chang Shi,