Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889786 | Journal of Microbiological Methods | 2011 | 5 Pages |
Abstract
The measurement of elemental selenium (Se0) is needed to assess the rate and magnitude of bacteria reduction of selenite or selenate. We have developed a spectrophotometric method for the measurement Se0 that is rapid and can be employed to measure the quantity of Se0 produced by bacterial cultures. This method employs the use of 1Â M Na2S to convert the insoluble elemental selenium to a red-brown solution and with this method there is a direct correlation between concentration of elemental selenium and the absorption at 500Â nm. To demonstrate the utility of this assay, we have followed the reduction of selenite to Se0 by Moraxella bovis, and by bacterial consortia in soil and water samples.
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Authors
Keka C. Biswas, Larry L. Barton, Wai Lok Tsui, Kevin Shuman, Jacquelyn Gillespie, Chioma S. Eze,