Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889837 | Journal of Microbiological Methods | 2010 | 5 Pages |
Abstract
Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.
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Authors
Kensuke Kataoka, Ursula E. Schoeberl, Kazufumi Mochizuki,