Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10889921 | Journal of Microbiological Methods | 2005 | 5 Pages |
Abstract
A protocol for direct sequencing of polymerase chain reaction (PCR) products from mycelia of Discula umbrinella, a fungal endophyte, using bufferless electrophoresis is described. This improved method allows researchers to conduct high-capacity screening of multiple gene regions for fungal endophytes applicable to microbial ecology and population genetic studies.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Susan D. Cohen,