| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10889942 | Journal of Microbiological Methods | 2005 | 4 Pages |
Abstract
Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.
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Authors
Tamimount Mohammadi, Henk W. Reesink, Christina M.J.E. Vandenbroucke-Grauls, Paul H.M. Savelkoul,