A comparative study of fluorescence-labelled enzyme activity methods for assaying phosphatase activity in phytoplankton. A possible bias in the enzymatic pathway estimations

We compared different fluorescence-labelled enzyme activity (FLEA) methods for assaying phosphatase activity in phytoplankton. Unfixed and liquid incubations are devised. We demonstrated that the presence of intracellular labelling was persistent, which could point out a source of bias in ectoenzymatic activities measurements based either on the FLEA or classical methods.