Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10890673 | Journal of Microbiological Methods | 2011 | 4 Pages |
Abstract
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
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Authors
Stina Rikke Jensen, Lisbeth Nielsen Fink, Carsten Struve, Claus Sternberg, Jens Bo Andersen, Jørn Brynskov, Ole Haagen Nielsen, Susanne Brix,