Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10890722 | Journal of Microbiological Methods | 2005 | 5 Pages |
Abstract
Here the cDNA-fluorescent amplified fragment length polymorphism (cDNA-FAFLP) technique, an improvement of cDNA-AFLP method, was used in order to analyse expression profiling in bacteria. The obtained results were validated by real-time PCR. This is the first report that validates the foreseen transcription pattern by cDNA-FAFLP with the application of real-time PCR in bacteria. This new protocol offers the possibility of quick and reliable analysis of transcription profiles, also avoids the problem linked with the use of radioisotopes, and allows a quick identification of genes differentially expressed in bacteria.
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Authors
Francesca Decorosi, Carlo Viti, Alessio Mengoni, Marco Bazzicalupo, Luciana Giovannetti,