Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10890770 | Journal of Microbiological Methods | 2005 | 5 Pages |
Abstract
A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Debby M. Klomberg, Hanneke A. de Valk, Johan W. Mouton, Corné H.W. Klaassen,