Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10957704 | Molecular and Cellular Probes | 2015 | 4 Pages |
Abstract
Vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. In this study, we developed a cross-priming amplification (CPA) assay coupled with vertical flow (VF) visualization for rapid and sensitive detection of V. parahaemolyticus. This assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). Small concentrations of V. parahaemolyticus (1.8 CFU/mL for pure cultures and 18 CFU/g for reconstituted samples) were detected within 1 h. CPA-VF can be applied at a large scale and can be used to detect V. parahaemolyticus strains rapidly in seafood and environmental samples, being especially useful in the field.
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Authors
Deshun Xu, Xiaofang Wu, Jiankang Han, Liping Chen, Lei Ji, Wei yan, Yuehua Shen,