| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10957785 | Molecular and Cellular Probes | 2005 | 5 Pages |
Abstract
Raw beef food products (raw ground beef and raw boneless beef) at 25 and 375Â g samples and bovine fecal samples at 2Â g were inoculated with 101, 103, 104, and 105 organisms of E. coli O157:H7 to test the sensitivity of this assay. Fourteen different foodborne bacteria (including E. coli O157:H7) and 19 various E. coli strains, obtained from the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) were tested for specificity. E. coli O157:H7 was detected at the level of 101 organisms in both 25 and 375Â g samples of raw ground and raw boneless beef products as well as 2Â g samples of bovine feces after pre-enrichment and concentration. None of the 14 foodborne bacteria screened for cross-reactivity was detected. All USDA E. coli strains confirmed to contain the eae gene were detected.
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Authors
Jay L.E. Ellingson, Jeff J. Koziczkowski, Jennifer L. Anderson, Steve A. Carlson, Vijay K. Sharma,