| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10962067 | Tuberculosis | 2014 | 5 Pages |
Abstract
This study confirmed that M. tuberculosis wild type PZAse was able to form homo-dimers in vitro. Homo-dimers showed a slightly lower specific PZAse activity compared to monomeric PZAse. PZAse dimers were dissociated into monomers in response to reducing conditions. Mass spectrometry analysis confirmed the existence of disulfide bonds (C72-C138 and C138-C138) stabilizing the quaternary structure of the PZAse homo-dimer.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
Daniel Rueda, Patricia Sheen, Robert H. Gilman, Carlos Bueno, Marco Santos, Victoria Pando-Robles, Cesar V. Batista, Mirko Zimic,