Article ID Journal Published Year Pages File Type
10962088 Tuberculosis 2013 12 Pages PDF
Abstract
We also present a phagocytosis assay in which we have minimised the impact of mycobacterial clumping, investigated the effect of various opsonisation techniques and established the correct usage of trypan blue to identify surface-bound organisms without counting dead cells. The same multiplicity of infection and serum conditions are optimal to demonstrate both internalisation and restriction of mycobacterial growth.
Related Topics
Life Sciences Immunology and Microbiology Applied Microbiology and Biotechnology
Authors
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