Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10962088 | Tuberculosis | 2013 | 12 Pages |
Abstract
We also present a phagocytosis assay in which we have minimised the impact of mycobacterial clumping, investigated the effect of various opsonisation techniques and established the correct usage of trypan blue to identify surface-bound organisms without counting dead cells. The same multiplicity of infection and serum conditions are optimal to demonstrate both internalisation and restriction of mycobacterial growth.
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Authors
David M. Lowe, Nonzwakazi Bangani, Meera R. Mehta, Dirk M. Lang, Adriano G. Rossi, Katalin A. Wilkinson, Robert J. Wilkinson, Adrian R. Martineau,