Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10962107 | Tuberculosis | 2014 | 5 Pages |
Abstract
Mycobacterium tuberculosis is the major causative agent of tuberculosis in humans. It is well known that Mycobacterium bovis and other species in the M. tuberculosis complex (MTC) can cause respiratory diseases as zoonosis. We analyzed the MTC isolates collected from tuberculosis patients from Japan in 2002 using a multiplex PCR system that detected cfp32, RD9 and RD12. A total of 970 MTC isolates that were representative of the tuberculosis cases throughout Japan, were examined using this method. As a result, 966 (99.6%) M. tuberculosis, two Mycobacterium africanum and two Mycobacterium canettii were identified using a multiplex PCR system, while no M. bovis was detected. Two isolates that lacked RD9 were initially considered to be M. canettii, but further analysis of the hsp65 sequence revealed them to be M. tuberculosis. Also two M. africanum were identified as M. tuberculosis using the â215 narG nucleotide polymorphism. Though PCR-linked methods have been used for a rapid differentiation of MTC and NTM, from our cases we suggest careful interpretation of RD based identification.
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Authors
Masako Ueyama, Kinuyo Chikamatsu, Akio Aono, Yoshiro Murase, Naoyuki Kuse, Kozo Morimoto, Masao Okumura, Takashi Yoshiyama, Hideo Ogata, Kozo Yoshimori, Shoji Kudoh, Arata Azuma, Akihiko Gemma, Satoshi Mitarai,