Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11011627 | International Journal of Hydrogen Energy | 2018 | 12 Pages |
Abstract
There is a growing global demand on bio-hydrogen production (BHP) using costless and wastes material. Herein we demonstrate the possibility to produce high yield of hydrogen using a new bacterial strain grown on acidic hydrolyzed cyanobacterial biomass as a costless carbon feedstock under various iron concentrations. We used E. coli DH701 mutant and new strain Brevibacillus invocatus SAR isolated from Assiut city soil samples. The mentioned new strain was identified morphologically, biochemically and by molecular analysis using 16S rDNA sequence. Limitation of iron induced BHP in tested cyanobacteria. Iron concentration (0.045Â mM) enhanced hydrogenase activity and cumulative hydrogen evolution in the investigated cultures. B. invocatus yielded 3.3Â mol H2/mole glucose and 3.8Â mol H2/mole reducing sugar (algal biomass), while the mutant strain yielded 1.78Â mol H2/mole glucose and 3.4Â mol H2/mole reducing sugar (algal biomass). Thus, the use of algal biomass induced higher potency of BHP especially at 0.045Â mM iron.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Sanaa M.F. Gad El-Rab, Awatief F. Hifney, Refat Abdel-Basset,