High-sensitivity glycated hemoglobin (HbA1c) aptasensor in rapid-prototyping surface plasmon resonance

Diabetes mellitus is one of the most common noncommunicable diseases in the world. Fasting blood glucose level is one of the diabetes diagnostic criteria, but it is readily affected by stress, exercise and many other factors. Glycated hemoglobin (HbA1c) is an important clinical index for diabetes and related diseases. In this study, a cheaper, rapid-prototyping, high-sensitivity angle-scanning surface plasmon resonance (SPR) was developed by fused deposition modeling (FDM) 3D printing technology for HbA1c detection. The sensor chip is based on an aptamer that has high affinity and high specificity to HbA1c. The results showed that this HbA1c-specific aptamer had high specificity for HbA1c, and the calculated KD was 6.13 × 10−8 M. The linear detection response of HbA1c appeared in the range of 18-147 nM, with a detection limit of 1 nM. This SPR HbA1c detection system could be a promising platform for developing clinical point-of-care diagnostic applications.