Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11016668 | Journal of Biotechnology | 2018 | 35 Pages |
Abstract
Laser Doppler Anemometry and Fast Fourier Transform analysis were used to identify characteristic flow frequencies under different agitation modes. Intermittent agitation resulted in a pattern of low intensity frequencies at reactor scale that could be controlled by varying three identified time components: rotational speed, interval and dwell times. A proof of concept biological study was undertaken, tuning the hydrodynamic environment through variation of dwell time based on the engineering study findings and a significant improvement in CM yield was obtained. This work introduces the concept of fine-tuning the physical hydrodynamic cues within a three-dimensional flow system to improve cardiomyocyte differentiation of iPSC.
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Authors
Jasmin J. Samaras, Bernardo Abecasis, Margarida Serra, Andrea Ducci, Martina Micheletti,