| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 11019566 | Protein Expression and Purification | 2019 | 12 Pages |
Abstract
The gene of mtl from the mussel Mytilus trossulus was cloned into pET-40b(+) expression vector. After expression in E. coli using designed MX-medium an instable soluble form of MTL was obtained. The developed isolation method of the recombinant protein in “semi-denatured” conditions allowed obtaining an active soluble form of the homogenous lectin from the mussel M. trossulus (r-MTL). Both of the lectins had similar antigenic and spatial structures.
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Authors
V.A. Golotin, A.P. Filshtein, I.V. Chikalovets, Kim N. Yu, V.I. Molchanova, O.V. Chernikov,