| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 11026036 | International Journal of Biological Macromolecules | 2018 | 41 Pages |
Abstract
The optimal extraction conditions with yield of 11.86â¯Â±â¯0.21% for extraction of crude polysaccharide from black mulberry fruit were obtained as extraction temperature 87â¯Â°C, extraction time 3â¯h and ratio of water to raw material 39â¯mL/g. Two purified fractions of BMP-1-1 and BMP-2-1 with average molecular weights of 615 and 405â¯kDa, respectively, were obtained by chromatography of DEAE-52 cellulose and Sephadex G-100. BMP-1-1 consisted of mannose, rhamnose, glucuronic acid, galactose and arabinose, BMP-2-1 consisted of rhamnose, glucuronic acid, galacturonic acid, galactose and arabinose. Crude BMP exhibited the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and the most effective scavenging activities against DPPH, ABTS and hydroxyl radical.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Wei Wang, Xuewen Li, Xiaowei Bao, Lei Gao, Yunxia Tao,