Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11026134 | International Journal of Biological Macromolecules | 2018 | 31 Pages |
Abstract
Due to the increasing application of oil and petroleum products, increased environmental contamination has become a matter of concern. Bio-desulfurization process may be used to eliminate sulfur from fossil fuels in the moderate condition. In this study, a thermophilic bacterium was isolated that was able to desulfurize dibenzothiophene. 16S rRNA sequencing indicated that this strain is related closely to Bacillus thermoamylovorans (97%). This strain grew in Basal salt medium containing DBT (100â¯mglâ1) as the only sulfur source, at 55°C and showed maximum growth (OD660â¯=â¯0.850) following 72â¯h incubation time. 2âhydroxybiphenyl was produced at the maximal concentration (26.13â¯Â±â¯0.12â¯mglâ1) at 72â¯h. Bio-desulfurization and growth rate factors were optimized using response surface methodology. Starch/Fe3O4 and starch/Fe nanoparticles were used for enhancement of BDS efficiency. The size of starch/Fe3O4 and starch/Fe nanoparticles were 20 and 30-40â¯nm, respectively, as described by using scanning electron microscope and transmission electron microscope. The results showed that the immobilized cells by starch/Fe3O4 and starch/Fe nanoparticles had higher desulfurization capacity, about 10% and 22% more, respectively. Also, BDS in a bioreactor in the presence of nanoparticles was increased 25% with respect of the process occurred in the flask.
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Authors
Narges Etemadi, Abbas Akhavan Sepahy, Ghasemali Mohebali, Fatemeh Yazdian, Meisam Omidi,