| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 11026199 | Experimental Gerontology | 2018 | 6 Pages |
Abstract
Recently, it has been suggested that oxytocin (OT) might play a role in the control of bone remodeling and in bone health of young and adult females. The purpose of this study was to evaluate the effect of osteogenic medium (OM) plus OT (OMâ¯+â¯OT; 100â¯nmol/L) on osteoblastic differentiation of bone marrow mesenchymal stem cells (BMMSCs) from cyclic adult (12â¯months old) and acyclic aging (24â¯months old) female Wistar rats. After 14â¯days, OMâ¯+â¯OT increased the oxytocin and oxytocin receptor in the BMMSCs from animals of both age groups relative to OM controls. Alkaline phosphatase activity was higher in the OMâ¯+â¯OT than OM group in BMMSCs from 24-month-old female rats. OMâ¯+â¯OT improved osteogenic differentiation, observed by anticipated mineralization and increased gene expression of bone morphogenetic protein 2, bone sialoprotein, osteopontin and osteocalcin in both aged relative to OM controls. These findings suggest a role for OT as an adjuvant to induce osteoblastic differentiation of BMMSCs from aged female rat.
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Authors
Leandro Figueiredo Santos, Monique Patricio Singulani, Camila Tami Stringhetta-Garcia, Sandra Helena Penha Oliveira, Antonio Hernandes Chaves-Neto, Rita Cássia Menegati Dornelles,