Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11029931 | Sensors and Actuators B: Chemical | 2018 | 6 Pages |
Abstract
MiRNA is reported as an important biomarker candidate and therapeutic target in a variety of diseases and cancers. However, ultrasensitive, straightforward, isothermal, low-cost and credible methods for the detection of miRNA from clinical specimens still remain challenges. Here, we establish an isothermal, highly sensitive and specific method for miRNA analysis based on a novel ligation-promoted hyperbranched rolling circle amplification (L-HRCA). This strategy exhibits 103-fold sensitivity enhancement compared to T4 ligase-assisted target-primer BRCA. The excellent specificity of L-HRCA allows for discriminating target miRNA from negative miRNAs. This method is applied to analyse the expression level of miR-21 in hepatocellular carcinoma. These results indicated that the L-HRCA strategy will become a promising tool to quantify miRNA in early clinical diagnostics.
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Authors
Xiaoe Zhang, Yinong Liu, Yiwen Yang, Junjie Huang, Haitao Wang, Zirui Zhu, Xi Wang, Pei Ma, Xin Zhou, Shaoru Wang, Xiang Zhou,