Limits of rapid log P determination methods for highly lipophilic and flexible compounds

•Different log P values were obtained for phenylalkanoic acids series including the pro-perfume Haloscent®D, depending on the chromatographic method used for log P estimation.•Molecular modelling suggested that log P variations may be due to the chromatographic conditions applied, responsible of specific conformations of the molecule in solution.•For flexible compounds, chromatographic methods have to be used with caution and considered as a good tool to estimate a log P range, that depends on the conformational state.

Lipophilicity is of crucial importance in many fields including pharmaceutical, environmental, cosmetic and food industries. Whereas different experimental strategies have been developed for rapid lipophilicity determination of new chemical entities, log P determination of highly lipophilic compounds is always challenging. In this study, three published chromatographic methods have been compared on a series of phenylalkanoic acids including the pro-perfume Haloscent®D (HD-C12). Different log P values were obtained depending on the chromatographic method used for log P estimation. Molecular modelling suggested that log P variations may be due to the chromatographic conditions applied (isocratic or gradient mode, ratio methanol/water in the mobile phase), responsible of specific conformations of the molecule in solution. Thus, for flexible compounds, published methods have to be used with caution and considered as a good tool to estimate a log P range, depending on the molecular conformational state.

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