Reusable and robust high sensitive non-enzymatic glucose sensor based on Ni(OH)2 nanoparticles

•Simple fabrication of robust and reusable non-enzymatic electrochemical glucose sensor.•Graphite electrode simply modified with nickel hydroxide nanoparticles in 30 s.•The sensor detects glucose in real sample with high sensitivity, selectivity and stability.•The sensor showed long-term stability over than 6 months.

Nickel hydroxide nanoparticles were successfully electrodeposited on graphite electrode (Gr/NiONP) and employed as a robust non-enzymatic glucose sensor. The results of cyclic voltammetry (CV) and chronoamperometry demonstrated that the Gr/NiONP electrode displayed high electrocatalytic activity toward glucose. The oxidation current is directly related to the glucose concentration from 1 μM to 15 mM. Besides, the glucose sensor displayed high sensitivity (2400 μA mM−1 cm−2) with a detection limit of 0.53 μM (S/N = 3) in basic solution. Moreover, the sensor showed excellent selectivity, reproducibility and stability properties. The relative standard deviation is 1.2% for 10 successive measurements in 16 μM glucose. Interestingly, the signal for glucose was maintained at 95% of its initial value even after 6 months of storage under ambient conditions. Gr/NiONP electrode has also been tested to detect glucose in human serum with satisfactory results.

Graphical abstractCVs of the bare Gr (a, b) and Gr/NiONP (c, d) electrode in absence (a, c) and presence (b, d) of 8 mM glucose in 0.1 M NaOH and scan rate 100 mV s−1.Figure optionsDownload full-size imageDownload as PowerPoint slide