| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1164458 | Analytica Chimica Acta | 2013 | 8 Pages |
•A magneto immunoassay for the detection of anti-transglutaminase antibodies (ATG2).•The ATG2 were captured by transglutaminase enzyme immobilized on magnetic beads.•The fluorescent response of the revelation system was related to ATG2.•The novel assay can discriminate correctly between celiac and non-celiac patients.•The novel assay is four times more sensitive than the commercial ELISA assay.
A magneto immunofluorescence assay for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The ATG2 were recognized by transglutaminase enzyme immobilized on the magnetic beads and then the immunological reaction was revealed by antibodies labeled with peroxidase. The fluorescent response of the enzymatic reaction with o-phenylenediamine and H2O2 as substrates was correlated with anti-transglutaminase titer, showing EC50 and LOD values of 1:11,600 and 1:74,500 of antibody titers, respectively. A total number of 29 sera samples from clinically confirmed cases of celiac disease and 19 negative control samples were tested by the novel magneto immunofluorescence assay. The data were submitted to the receiver-operating characteristic plot (ROC) analysis which indicated that 8.1 U was the most effective cut-off value to discriminate correctly between celiac and non-celiac patients. The immunofluorescence assay exhibited a sensitivity of 96.6%, a specificity of 89.5% and an efficiency 93.8% compared with the commercial optical ELISA kit.
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