Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1164698 | Analytica Chimica Acta | 2013 | 7 Pages |
•Anti-ricin single domain antibodies (sdAb) were self-assembled on quantum dots (QDs).•Conjugates were prepared using dihydrolipoic acid-capped CdSe–ZnS core–shell QDs.•The sdAb–QD conjugates functioned in fluoroimmunoassays for ricin detection.•The conjugates provided signal amplification in surface plasmon resonance assays.•Conjugates provided sensitive detection compared to unconjugated sdAb reporters.
The combination of stable biorecognition elements and robust quantum dots (QDs) has the potential to yield highly effective reporters for bioanalyses. Llama-derived single domain antibodies (sdAb) provide small thermostable recognition elements that can be easily manipulated using standard DNA methods. The sdAb was self-assembled on dihydrolipoic acid (DHLA) ligand-capped CdSe–ZnS core–shell QDs made in our laboratory through the polyhistidine tail of the protein, which coordinated to zinc ions on the QD surface. The sdAb–QD bioconjugates were then applied in both fluorometric and surface plasmon resonance (SPR) immunoassays for the detection of ricin, a potential biothreat agent. The sdAb–QD conjugates functioned in fluoroimmunoassays for the detection of ricin, providing equivalent limits of detection when compared to the same anti-ricin sdAb labeled with a conventional fluorophore. In addition, the DHLA-QD–sdAb conjugates were very effective reporter elements in SPR sandwich assays, providing more sensitive detection with a signal enhancement of ∼10-fold over sdAb reporters and 2–4 fold over full sized antibody reporters. Commercially prepared streptavidin-modified polymer-coated QDs also amplified the SPR signal for the detection of ricin when applied to locations where biotinylated anti-ricin sdAb was bound to target; however, we observed a 4-fold greater amplification when using the DHLA-QD–sdAb conjugates in this format.
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