| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1164831 | Analytica Chimica Acta | 2013 | 5 Pages |
•RBCs were migrated faster than WBCs and blood pellets by laser photophoresis.•Photophoretic efficiency of RBC and WBC was simulated by the Mie scattering theory.•Spontaneous orientation of RBC parallel to the migration direction was elucidated.•Laser photophoretic separation of RBC and WBC was possible in a tip flow system.
Laser photophoretic migration behavior of human blood cells in saline solution was investigated under the irradiation of Nd:YAG laser beam (532 nm) in the absence and the presence of the flow in a fused silica capillary. Red blood cells (RBC) were migrated faster than white blood cells (WBC) and blood pellets to the direction of propagation of laser light. The observed photophoretic velocity of RBC was about 11 times faster than those of others. This was understood from the larger photophoretic efficiency of RBC than that of WBC, which was simulated based on the Mie scattering theory. Furthermore, it was found that, during the photophoretic migration, RBCs spontaneously orientated parallel to the migration direction so as to reduce the drag force. Finally, it was demonstrated that RBC and WBC were separated in a micro-channel flow system by the laser photophoresis.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
