Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1165011 | Analytica Chimica Acta | 2013 | 5 Pages |
A unique analytical method using nanoparticles and laser-induced fluorescence microscopy (LIFM) was developed to determine enrofloxacin in this work. For sample pretreatment, two different kinds of particles, i.e., synthesized dye-doped core–shell silica nanoparticles and magnetic micro-particles (MPs), were used for fluorescent tagging and concentrating the enrofloxacin, respectively. The antibody of enrofloxacin was immobilized on the synthesized FITC-doped core–shell nanoparticles, and the enrofloxacin target was extracted by the MPs. At this moment, the average number of antibodies on each core–shell silica nanoparticle was ∼0.9, which was determined by the fluorescence ratiometric method. The described method was demonstrated for a meat sample to determine enrofloxacin using LIFM, and the result was compared with enzyme-linked immunosorbent assay (ELISA). The developed technique allowed the simplified analytical procedure, improved the detection limit about 54-fold compared to ELISA.
Graphical abstractLaser induced fluorescence microscopy and core–shell nanoparticles to determine antibiotics.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► An analytical method using two different nanoparticles to determine antibiotics. ► Dye-doped core–shell silica nanoparticles were used for fluorescent tagging while magnetic particles were used for concentrating the enrofloxacin. ► Improvement of the detection limit about 54-fold compared to ELISA.