| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1165234 | Analytica Chimica Acta | 2013 | 8 Pages |
•Hollow fiber microextraction based on supramolecular solvents was developed.•Supramolecular solvent was produced from coacervation of decanoic acid and Bu4N+.•The method was introduced for extraction of benzodiazepines from aqueous solutions.•Several parameters affecting extraction efficiency were investigated and optimized.•HPLC-DAD was applied for the determination of the drugs in fruit juice and urine samples.
A new, efficient, and environmental friendly hollow fiber liquid phase microextraction (HF-LPME) method based on supramolecular solvents was developed for extraction of five benzodiazepine drugs. The supramolecular solvent was produced from coacervation of decanoic acid aqueous vesicles in the presence of tetrabutylammonium (Bu4N+). In this work, benzodiazepines were extracted from aqueous samples into a supramolecular solvent impregnated in the wall pores and also filled inside the porous polypropylene hollow fiber membrane. The driving forces for the extraction were hydrophobic, hydrogen bonding, and π-cation interactions between the analytes and the vesicular aggregates. High-performance liquid chromatography with photodiode array detection (HPLC-DAD) was applied for separation and determination of the drugs. Several parameters affecting the extraction efficiency including pH, hollow fiber length, ionic strength, stirring rate, and extraction time were investigated and optimized. Under the optimal conditions, the preconcentration factors were obtained in the range of 112–198. Linearity of the method was determined to be in the range of 1.0–200.0 μg L−1 for diazepam and 2.0–200.0 μg L−1 for other analytes with coefficient of determination (R2) ranging from 0.9954 to 0.9993. The limits of detection for the target benzodiazepines were in the range of 0.5–0.7 μg L−1. The method was successfully applied for extraction and determination of the drugs in water, fruit juice, plasma and urine samples and relative recoveries of the compounds studied were in the range of 90.0–98.8%.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
