Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1165275 | Analytica Chimica Acta | 2013 | 7 Pages |
•A novel CE strategy combined chitosan trapping and CTAB assisted sample stacking.•Validation parameters of this method were investigated and satisfactory.•The method was used to detect organic acids in real Plateau alfalfa roots samples.
In this paper, four organic acids constituents of Plateau alfalfa roots have been identified and detected by a novel capillary electrophoresis (CE) strategy which combined chitosan (CS) trapping and cetyltrimethyl ammonium bromide (CTAB) assisted sample stacking. Under the optimized condition, organic acids, i.e., aconitic acid, gallic acid, citric acid and l-malic acid were concentrated and separated within 3 min. Validation parameters of this method (such as detection limits, linearity and precision) were also investigated and the limit of detection (LOD) was 2.41–53.9 ng mL−1. Linearity was obtained over the magnitude range of 5–4000 ng mL−1 approximately for different organic acids and 3 × 102–1.5 × 104 folds enrichment was achieved. The method has been applied to the determination of organic acids in roots of normal grown Plateau alfalfa and stressing affected Plateau alfalfa. Satisfactory results and recoveries were obtained in the analysis without costly and complicated sample pretreatment.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide