| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1165282 | Analytica Chimica Acta | 2012 | 9 Pages |
Lychnophora salicifolia Mart., which occurs in the Brazilian Cerrado in the states of Bahia and Minas Gerais as well as in the southeast of the state of Goiás, is the most widely distributed and also the most polymorphic species of the genus. This plant is popularly known to have anti-inflammatory and analgesic activities. In this work, we have studied the variation in terms of polar metabolites of ninety-three Lychnophora salicifolia Mart. specimens collected from different regions of the Brazilian Cerrado. Identification of the constituents of this mixture was carried out by analysis of the UV spectra and MS data after chromatographic separation. Twenty substances were identified, including chlorogenic acid derivatives, a flavonoid C-glucoside, and other sesquiterpenes. The analytical method was validated, and the reliability and credibility of the results was ensured for the purposes of this study. The concentration range required for analysis of content variability within the analyzed group of specimens was covered with appropriate values of limits of detection and quantitation, as well as satisfactory precision and recovery. A quantitative variability was observed among specimens collected from the same location, but on average they were similar from a chemical viewpoint. In relation to the study involving specimens from different locations, there were both qualitative and quantitative differences among plants collected from different regions of Brazil. Statistical analysis revealed that there is a correlation between geographical localization and polar metabolites profile for specimens collected from different locations. This is evidence that the pattern of metabolites concentration depends on the geographical distribution of the specimens.
Graphical abstractStatistical analysis revealed that there is a correlation between geographical localization of Lychnophora salicifolia samples and polar metabolites profile for specimens collected from different locations.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A new HPLC-DAD-MS method for analysis of the crude extract of L. salicifolia leaves. ► Twenty substances were identified. ► Ninety-three wild specimens were analyzed. ► A correlation between geographic isolation and decrease of the chemical similarity. ► The pattern of metabolites depends on the geographical distribution of the specimens.
