Article ID Journal Published Year Pages File Type
1165544 Analytica Chimica Acta 2013 6 Pages PDF
Abstract

A sensitive method based on programmable temperature vaporization large volume injection coupled to gas chromatogram and high-resolution mass spectrometry (PTV-GC–HRMS) has been developed for the determination of ultra trace levels of dioxin-like polychlorinated biphenyls (DL PCBs) in small amounts of human blood. Blood samples (1 mL) were first extracted by column extraction and then purified with column chromatorgraphies. Final extracts (20 μL) were introduced to the PTV injector under the solvent vent mode and detected by GC–HRMS (SIM mode). PTV parameters were observed by changing one factor at a time (practical conditions: vent flow: 50 mL min−1, vent pressure: 0 kPa and vent time: 0.1 min), recoveries of most PCB congeners ranged from 55.1% to 108%, and method detection limits were in the range of 0.11–1.63 pg g−1.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We developed and validated a PTV-GC–HRMS method to measure low level DL-PCBs. ► Liquid nitrogen was used in PTV external accessory equipment as fast cooling system. ► PTV's high-throughput and HRMS’ high sensitivity and selectivity are well combined.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , , , , , , ,