Article ID Journal Published Year Pages File Type
1165774 Analytica Chimica Acta 2012 8 Pages PDF
Abstract

A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites toward the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48 pg mL−1, which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Sensitive, early detection of cytokines can help in identifying perinatal diseases. ► Appropriately grafted dendrimer on the biosensor leads to better antibody orientation. ► Significantly improved detection limit and sensitivity has been shown for TNF-α. ► Platform approach is adaptable to both capture and detection, and for a wide variety of cytokines.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , ,